RNA

Part:BBa_K3120000:Design

Designed by: Ruoyi Xue   Group: iGEM19_BM-AMU   (2019-08-06)


E-cadherin's 20nt target sequence+sgRNA scaffold


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene target site which selected must be a DNA sequence of about 20 nt that satisfy two conditions: 1.This sequence is unique in the genome. 2.The gene target site is immediately upstream of the PAM (Protospacer Adjacent Motif) region.


Source

Designed according to the CDS sequence of the E-cadherin gene and submitted to The Beijing Genomics Institute for synthesis.

References